Microscope images of stained sperm

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  • 09.12.2018
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The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series of morphological events. Incubation of the sperm cells in the presence of MTT resulted after a few min in a diffuse staining of the midpiece of the sperm cells. Upon further incubation the staining of the midpiece became more intense, and gradually the formation of packed formazan granules became more visible. At the same time, a small formazan stained granule appeared medially on the sperm head, which increased in size during further incubation.
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Microscopic analysis of MTT stained boar sperm cells

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Sperm Morphology

The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology ART. It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope and whether the male partner's age affects these vacuoles. The criteria for the sperm-head vacuoles were those given in the World Health Organization manual. For the analysis of the age factor, the participants were divided into the following groups: years, years, years, years, and years.
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Observation of sperm-head vacuoles and sperm morphology under light microscope

Skip to search form Skip to main content. The measurement or evaluation and clinical significance of human sperm morphology has always been and still is a controversial aspect of the semen analysis for the determination of a male's fertility potential. The evaluation of sperm size, shape and morphological smear characteristics should be assesed by carefully observing a stained sperm sample under a microscope. View on IEEE. Save to Library.
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